Figure 3
From: Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans
Manganese sensitivity assay for wild-type R1 and the mntE- mutant. (A) Wild-type R1 (white bars), mntE- (black bars), and WT revertant (gray bars) were cultured on TGY plates overlaid with filter discs saturated with 1 M solutions of various cations. The zone of inhibition was measured from the edge of the disc after three days. *P < 0.01. ND, not determined. (B) The inhibition zone of R1 and mntE-. Cells were cultured on TGY plates overlaid with filter discs saturated with 1 M manganese chloride. (C) Wild-type R1 and mntE- were inoculated in TGY supplemented with different concentrations of manganese chloride. The OD600 values were determined after 12 h. Data represent the means ± standard deviations of three independent experiments.