Figure 2

Minimum spanning tree of 330 A. fumigatus isolates based on categorical analysis of 10 VNTRs. Each circle represents a unique genotype. The diameter of each circle corresponds to the number of isolates with the same genotype. Genotypes connected by a shaded background differ by a maximum of 2 of the 10 VNTR markers and could be considered a "clonal complex". Thick connecting lines represent one marker difference; regular connecting lines represent two marker differences; thick interrupted lines represent three differences; thin interrupted lines represent four or more differences. The length of each branch is also proportional to the number of difference. Each epidemiological situation is represented by a specific colour: red for isolates collected during an epidemiological survey conducted in the same duck farm in 2007 and 2008 in Sarthe region, France; salmon pink for isolates collected during an epidemiological survey conducted in another single duck farm in 2007 and 2008 in Sarthe region, France; navy blue for isolates collected during an epidemiological survey in a chicken farm in 2008 in Guangxi province, China; light blue for isolates collected during an epidemiological survey in a duck farm in 2008 in Guangxi province, China; green for environmental isolates collected in 2009 in a turkey hatchery in Maine et Loire region, France and yellow for unrelated isolates. Large ellipses correspond to the three major clusters (with the colour of the majority of the genotypes).