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Figure 1 | BMC Microbiology

Figure 1

From: Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification

Figure 1

Effect of increasing concentration differences between targets in multiplex qPCR reactions. Dilution series of multicopy targets (A-C) or internal control target cry1(D-F) were made in the presence of the other targets detected in each qPCR at a constant concentration near the detection limit. Triplicate multiplex qPCR measurements were performed and mean Cq values with 95% confidence limits are shown for each target.

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BMC Microbiology

ISSN: 1471-2180

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