Skip to main content

Table 4 Sensitivities and specificities of multiplex real-time PCR for detection of S. pneumoniae and H. influenzae.

From: Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis

Species

Reference test

Detection limit of the assay

Cutoff 105 copies/mL

  

Sensitivity

Specificity

PPVa

NPVb

Sensitivity

Specificity

PPV

NPV

S. pneumoniae

BAL culture, blood culture and urinary antigen test

95%

(20/21)

75%

(101/135)

37%

(20/54)

99%

(101/102)

90%

(19/21)

80%

(108/135)

41%

(19/46)

98%

(108/110)

 

BAL culture, blood culture and urinary antigen tes + lytA PCR

91%

(43/47)

89%

(97/109)

78%

(43/55)

96%

(97/101)

79%

(37/47)

95%

(104/109)

88%

(37/42)

91%

(104/114)

H. influenzae

BAL culturec

90%

(28/31)

65%

(81/125)

39%

(28/72)

96%

(81/84)

81%

(25/31)

85%

(106/125)

57%

(25/44)

95%

(106/112)

 

BAL culturec + fucK PCRd

93%

(69/74)

96%

(79/82)

96%

(69/72)

94%

(79/84)

63%

(47/74)

100.0%

(82/82)

100%

(47/47)

75%

(82/109)

  1. a Positive predictive value
  2. b Negative predictive value
  3. c Blood culture were also performed for H. influenzae but all were negative
  4. d fucK PCR was performed in the PCR positive and culture negative samples
  5. Analysis of bronchoalveolar lavage from 156 adults with lower respiratory tract infection.
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us