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Table 3 Comparison of reference tests with quantitative multiplex PCR (qmPCR).

From: Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis

Results

  

Reference testsa

qmPCRb

No. of patients

No. on antibiotic treatment

A.

   

Spn & Hi

Spn & Hi

1

1

Spn & Hi

Hi

1

1

Spn

Spn & Hi

5

4

Spn

Spn

14

6

-

Spn

20

15

-

Spn & Hi

9

7

Hi

Spn & Hi

5

5

Hi

Hi

21

12

Hi

-

3

3

-

Hi

30

26

-

-

47

24

B.

   

Spn

Hi

1

 

Spn

Spn

1

 

Hi

Spn & Hi

1

 

Hi

Hi

2

1

-

Spn

3

1

-

Spn & Hi

3

 

-

Hi

4

 

-

-

16

1

  1. a Blood culture, urinary antigen test, and BAL culture for S. pneumoniae; blood culture and BAL culture for H. influenzae.; -, negative test result.
  2. b Spn9802+ was considered as S. pneumoniae+; P6+ was considered as H. influenzae+.
  3. Results of reference tests and qmPCR for Streptococcus pneumoniae (Spn) and Haemophilus influenzae (Hi) applied to bronchoalveolar lavage (BAL) samples in 156 patients with lower respiratory tract infection (A) and in 31 control patients (B).
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BMC Microbiology

ISSN: 1471-2180

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