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Table 1 Oligonucleotide primers and probes for detection of S. pneumoniae, H. influenzae and N. meningitidis.

From: Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis

  Sequence (5' to 3')a Positions in target gene
S. pneumoniae   
Spn9802 F 5'-A GTC GTT CCA AGG TAA CAA GTC T-3' 3370-3392
Spn9802 R 5'-AC CAA CTC GAC CAC CTC TTT-3' 3525-3506
Spn9802 FAM 5'-FAM-aTc AGa TTg CTg ATa AAa CgA-BHQ1-'3  
H. influenzae   
Hi P6 F 5'-CCA GCT GCT AAA GTA TTA GTA GAA G-3' 302-326
Hi P6 R 5'-TTC ACC GTA AGA TAC TGT GCC-3' 477-457
Hi P6 JOE 5'-JOE- CAg ATg CAg TTg AAg GTt Att tAG-BHQ1-'3  
N. meningitidis   
ctrA F 5'-GCTGCGGTAGGTGGTTCAA-3' 617-635
ctrA R 5'-TTGTCGCGGATTTGCAACTA-3' 727-708
ctrA ROX 5'-ROX-CATTGCCACGTGTCAGCTGCACAT- BHQ1-'3  
  1. a Positions with lower case letters indicate locked nucleic acid [27].