Western blotting analysis of anti-MtsA antibodies in infected sera from Kunming mice with S. iniae HD-1 infection. SDS-PAGE analysis showing the purification results of MtsA. The gel was transferred to a nitrocellulose membrane and blotted with infected sera from mice. The gels were stained with Coomassie brilliant blue. Lane 1, molecular mass marker; lane 2, E. coli with control pet-32a-c (+) vector; lane 3, E. coli lysate containing MtsA (approximately 49.5-kDa); lane 4, purified MtsA (approximately 49.5-kDa); lanes 5~7, western blot results of infected sera, lanes 8~10, western blot results of control sera; lanes 5 and 8, western blot results of E. coli with the control vector; lanes 6 and 9, E. coli lysate containing MtsA, and lanes 7 and 10, purified MtsA (approximately 49.5-kDa).