Figure 4From: A solute-binding protein for iron transport in Streptococcus iniaeSDS-PAGE and western blotting analysis of expressed and purified MtsA. Lanes 1~4, SDS-PAGE showing the purification results of MtsA. The gels were stained with Coomassie brilliant blue. Lane 1, molecular mass marker; lane 2, E. coli with control pet-32a-c (+) vector; lane 3, E. coli lysate containing MtsA (approximately 49.5-kDa); lane 4, purified MtsA (approximately 49.5-kDa). Lanes 5~7, western blotting results of purified MtsA. Lane 5, E. coli with control pet-32a-c (+) vector; lane 6, E. coli lysate containing MtsA (approximately 49.5-kDa); lane 7, purified MtsA (approximately 49.5-kDa).Back to article page