Analysis of the lipoprotein sequence patterns of MtsA by ScanProsite and the western blotting. (A) The mtsABC lipoprotein was assessed by ScanProsite. The results showed that amino acid residues D1 to D24 (MFKKISLAFAMLLSIFCITACSSQ) hit G+LPPv2 pattern, and amino acid residues D17 to D21 (CITAC) hit PS51257 pattern. The symbol "<" indicates that the pattern is restricted to the N terminus, and X is any amino acid. (B) Western blotting analysis results of the lipoproteins extracted with Triton X-114.