Figure 8

Imago of stage W1. Overview (A) and detailed images of different optical sections (B - E) of the midgut of a young worker shortly after eclosion (W1) by confocal laser scanning microscopy (for further information regarding the composition of the figure see legend of Fig. 1). In the overview (A) the proventriculus can be seen on the right side of the midgut. The number of not-infected cells with larger nuclei is increased in comparison to the late pupae stages (Fig. 7). Still there are bacteria in cells which do not resemble typical bacteriocytes (e.g. white arrows in figure part D). Green label: The Blochmannia specific probe Bfl172-FITC; red label: SYTO Orange 83. The scale bars correspond to 220 μM (A) and 35 μM (B - E), respectively.