Figure 7
Pupa of stage P3. Overview (A, red stained Malpighian tubules are visible on the top of the midgut) and detailed images of different optical sections (B - E) of the midgut of a pupa immediately before eclosion (P3) by confocal laser scanning microscopy (for further information regarding the composition of the figure see legend of Fig. 1). In comparison to P2 (see Fig. 6), a slight increase in bacteria-free midgut cells with large nuclei can be observed. Green label: The Blochmannia specific probe Bfl172-FITC; red label: SYTO Orange 83. The scale bars correspond to 220 μM (A) and 35 μM (B - E), respectively.