Table 2 Oligonucleotide primers used in this study.
Oligo name | Sequence (5'-3'). Restriction site underscored | Target gene |
|---|---|---|
SOE PCR | Â | Â |
VC1785A | GAGCTC AATGGTGCATCGGCATATTCT | vefA (A-D) |
VC1785B | CAGCGATGATGGCGTGATTA | Â |
VC1785C | TAATCACGCCATCATCGCTGGGATGT TCTCCTATGTCTTGT | Â |
VC1785D | TCTAGA CGCGCACATAACGCTGTTC | Â |
VC1809A | CTGCAG TGAGAGCAAGGGAAGTGATCGT | vefB(A-D) |
VC1809B | CGAGAGCTGGTTTACTTGTGTG | Â |
VC1809C | CACACAAGTAAACCAGCTCTCGGTGCATGATTGTCAAGTCATGCA | Â |
VC1809D | GAGCTC GATGGCTATGAATTAGCTCAGGA | Â |
Flanking | Â | Â |
VC1785FF | GATGCTTCTATTACTCGGTT | vefA (F-R) |
VC1785FR | TCACCGCTGCTGCGTTAA | Â |
VC1809FF | GATTGATAGTAACAACACGCG | vefB (F-R) |
VC1809FR | GTAATGCGCTATTGCTAAGTG | Â |
VPI2attF | AGAGTGAAAGTCGCCAAAGC | attB (F-R) |
VPI2attR | GGGTGCAATTTCGCATGTTGC | Â |
Complements | Â | Â |
VC1758CF | GAGCTC GAGTCCTCATGCTCTAGCCAG | intV2 (F-R) |
VC1758CR | TCTAGA GGCATGCTGGTGTGTTACTAC | Â |
VC1785CF | GAGCTC GCTTTGAATATAGGTAAGGGACTG | vefA (F-R) |
VC1785CR | TCTAGA CTATAGTACATGACGCATGTATAATC | Â |
Real-Time PCR | Â | Â |
VPI2attBQF | GATTCGGTGAGTTGTCCGAGT | attB (VPI-2) (F-R) |
VPI2attBQR | GTGTTGGTGCAATGCTCAGTC | Â |
VC1758QF | TGCATGATCTTATACTCACCG | intV2 (F-R) |
VC1758QR | ACGTGCTCGCGGTTCATCTTC | Â |
VC1785QF | CCGCACTAAGCCGTTCAGCAA | vefA (F-R) |
VC1785QR | CCATCCACTCATCCACTTCGC | Â |
VC1809QF | CTGAGAGGTGTGAATATGCCAG | vefB (F-R) |
VC1809QR | GTGATCCGTTGAGCAATCCAC | Â |
VC0432QF | TGTATGATATTGCGCCTGTCACAC | mdh(F-R) |
VC0432QR | CCAGAACCACATCCGCACCTTC | Â |