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Table 1 Growth and morphotype switching of 3 isogenic morphotypes derived from 5 B. pseudomallei isolates following incubation in low oxygen and anaerobic conditions

From: Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro

 

Atmospheric conditions during incubation at 37°C

Starting type

Air for 4 days (control)

5-15% oxygen for 14 days

Reincubated in air for 4 days following anaerobic conditions for 14 days

 

Mean colony count, (range)

*Morphotype, % (range)

Mean % colony count compared with control in air (range)

*Morphotype, % (range)

Mean % colony count compared to control in air (range)

*Morphotype, % (range)

I (parental)

101

(93-106)

I

100%

92%

(78-108%)

I

100%

86%

(57-138%)

I

100%

II

90

(62-150)

II

100%

91%

(72-109%)

II

100%

95%

(66-127%)

II

100%

III

123

(110-141)

III

89%

(81-98%)

98%

(78-107%)

III

89%

(81-99%)

80%

(48-94%)

III

17%

(0-85%)

  

I or

II

11%

(2-19%)

 

I or II

11%

(1-19%)

 

I or

II

83%

(15-100%)

  1. The data represents the mean of 5 B. pseudomallei isolates for each morphotype. The range reflected variation of % colony count between isolates. *% Morphotype was the proportion of each morphotype on the plate. Morphotype switching was observed for type III (starting type) to either type I (isolates K96243, 164, B3 and B4) or to type II (isolate 153).