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Table 1 Growth and morphotype switching of 3 isogenic morphotypes derived from 5 B. pseudomallei isolates following incubation in low oxygen and anaerobic conditions

From: Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro

  Atmospheric conditions during incubation at 37°C
Starting type Air for 4 days (control) 5-15% oxygen for 14 days Reincubated in air for 4 days following anaerobic conditions for 14 days
  Mean colony count, (range) *Morphotype, % (range) Mean % colony count compared with control in air (range) *Morphotype, % (range) Mean % colony count compared to control in air (range) *Morphotype, % (range)
I (parental) 101
(93-106)
I 100% 92%
(78-108%)
I 100% 86%
(57-138%)
I 100%
II 90
(62-150)
II 100% 91%
(72-109%)
II 100% 95%
(66-127%)
II 100%
III 123
(110-141)
III 89%
(81-98%)
98%
(78-107%)
III 89%
(81-99%)
80%
(48-94%)
III 17%
(0-85%)
   I or
II
11%
(2-19%)
  I or II 11%
(1-19%)
  I or
II
83%
(15-100%)
  1. The data represents the mean of 5 B. pseudomallei isolates for each morphotype. The range reflected variation of % colony count between isolates. *% Morphotype was the proportion of each morphotype on the plate. Morphotype switching was observed for type III (starting type) to either type I (isolates K96243, 164, B3 and B4) or to type II (isolate 153).