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Table 2 Abundance differences of Y. pestis proteins profiled in membrane fractions of iron-rich vs. iron-starved cells

From: Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation

Spot No
a)
Gene locus
b)
gene name
c)
Protein description c) Subc. Loc.
d)
Fur/RyhB e) Mascot Score f) exp Mr (Da) exp pI 26°C, Vs (-Fe) g) 26°C, Vs(+Fe) h) 26-ratio -Fe/+Fe i) 26°C P-value j) 37-ratio -Fe/+Fe k)
94 y0032 lamB Maltoporin OM   331 48645 [4.95 - 5.09] 0.76 1.49 0.516 0.000 1.27
95 y0543 hmuR hemin outer membrane receptor OM Fur 1064 76570 5.05 0.25 0.10 2.600 0.000 4.665
96 y0850 - putative iron/chelate outer membrane receptor OM Fur 57 70302 [5.5 - 6.0] 1.54 0.22 6.978 0.000 2.430
97 y1355 - hypothetical inner membrane protein y1355 U   53 22715 5.59 0.32 0.57 0.560 0.000 0.820
98 y1577 fadL long-chain fatty acid transport protein (OM receptor) OM   1008 51392 [4.77 - 4.87] 0.37 0.81 0.460 0.000 0.370
99 y1632 nuoC NADH dehydrogenase I chain C, D CY   654 68079 [5.79 - 5.9] 0.07 0.18 0.367 0.000 0.578
100 y1682 ompX outer membrane protein X OM   389 18271 5.31 5.65 3.08 1.859 0.000 0.557
101 y1919 arnA bifunctional UDP-glucuronic acid decarboxylase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase U   346 72392 [5.86 - 5.92] 0.76 0.20 3.748 0.000 > 20
102 y2404 psn pesticin/yersiniabactin outer membrane receptor OM Fur 148 67582 [5.20 - 5.45] 6.80 1.46 4.862 0.000 2.656
103 y2556 fcuA ferrichrome receptor, TonB dependent OM Fur 801 76097 [5.64 - 5.94] 0.20 0.18 1.070 0.710 0.860
104 y2633 ysuR outer membrane iron/siderophore receptor OM Fur 202 73135 6.30 0.11 0.04 2.790 0.001 N.D.
105 y2735 ompA outer membrane porin A, N-t. fragment OM   686 34018 [5.52 - 5.75] 5.05 0.70 7.245 0.000 3.390
106 y2872 yiuR putative iron/siderophore outer membrane receptor OM Fur 133 67256 5.55 0.65 0.29 2.260 0.000 N.D.
107 y2966 ompC outer membrane porin protein C OM   1110 43707 [4.78 - 4.88] 2.18 1.45 1.500 0.010 0.487
108 y2980 yfaZ hypothetical protein y2980 CM   96 20054 5.48 0.30 0.66 0.459 0.000 0.202
109 y2983 phoE putative outer membrane porin OM   65 41703 [4.94 - 5.22] - 14.60 < 0.05 N.D. < 0.05
110 y3674 - putative type VI secretion system protein U   350 63614 [5.52 - 5.58] 0.72 0.44 1.620 0.002 N.D.
  1. a) spot number as denoted in Figure 3; b) protein accession number and locus tag as listed in Y. pestis KIM genome database (NCBI); c) gene name and protein description from the KIM database or a conserved E. coli K12 ortholog http://www.ecocyc.org, if >65 pct. sequence identity; d) subcellular localization based on PSORTb data: CY, cytoplasm; CM: inner membrane; OM: outer membrane; U: unknown; e) proven or putative regulation by Fur or a Fur-dependent small RNA (e.g. RyhB); f) highest Mascot score for a protein from LC-MS/MS or MALDI data; g) Vs (-Fe): average spot volume (n ≥ 3) in 2D gels for iron-depleted growth conditions at 26°C as shown in Figure 3; h) Vs (+Fe): average spot volume (n ≥ 3) in 2D gels for iron-supplemented growth conditions at 26°C; i) spot volume ratio (-Fe/+Fe) at 26°C, N.D.: not determined; -: no spot detected; j) two-tailed t-test p-value for spot abundance change at 26°C, 0.000 stands for < 0.001; k) average spot volume ratio (-Fe/+Fe) at 37°C; additional data for the statistical spot analysis at 37°C are part of Additional Table 1. Experimental pI values span a pH range because proteins were visualized as spot trains.