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Table 3 Comparison of pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP for the detection of E. ruminantium in various field samples

From: Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium

   No. of samples:
Sample type Origin (Site/Country) Tested pCS20 PCRa positive pCS20 real-time PCR positive pCS20 LAMP positive sodB LAMP positive
Bovine blood Butaleja/Uganda 50 0 NDb 0 0
  Petauke/Zambia 50 0 ND 0 0
  Serengeti/Tanzania 50 0 ND 0 0
Goat blood Chama/Zambia 35 0 ND 0 0
Lamb's blood Kerr Seringe/The Gambia 19 2 2 2 2
Sheep bloodc NAd 4 4 4 4 4
Tick;Amblyomma variegatum Amuria/Uganda 20 (15/5)e 2 (2/0) 4 (4/0) 4 (4/0) 4 (4/0)
  Butaleja/Uganda 20 (18/2) 0 1 (1/0) 1 (1/0) 1 (1/0)
  Dokolo/Uganda 20 (12/8) 1 (1/0) 2 (2/0) 1 (1/0) 1 (1/0)
  Kaberamaido/Uganda 20 (14/6) 0 1 (0/1) 1 (0/1) 1 (0/1)
  Pallisa/Uganda 20 (10/10) 2 (2/0) 2 (2/0) 2 (2/0) 2 (2/0)
  Soroti/Uganda 20 (17/3) 2 (2/0) 2 (2/0) 2 (2/0) 2 (2/0)
  Tororo/Uganda 20 (10/10) 1 (1/0) 1 (1/0) 1 (1/0) 1 (1/0)
  Subtotal for tick samples 140 (96/44) 8 (8/0) 13 (12/1) 12 (11/1) 12 (11/1)
  1. aPCR was performed using KAPA Blood PCR kit.
  2. bND, not done.
  3. cBlood samples from sheep experimentally infected with E. ruminantium were used as positive controls.
  4. dNA, not applicable.
  5. eTotal no. of ticks (No. of male ticks/No. of female ticks).