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Table 3 Comparison of pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP for the detection of E. ruminantium in various field samples

From: Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium

  

No. of samples:

Sample type

Origin (Site/Country)

Tested

pCS20 PCRa positive

pCS20 real-time PCR positive

pCS20 LAMP positive

sodB LAMP positive

Bovine blood

Butaleja/Uganda

50

0

NDb

0

0

 

Petauke/Zambia

50

0

ND

0

0

 

Serengeti/Tanzania

50

0

ND

0

0

Goat blood

Chama/Zambia

35

0

ND

0

0

Lamb's blood

Kerr Seringe/The Gambia

19

2

2

2

2

Sheep bloodc

NAd

4

4

4

4

4

Tick;Amblyomma variegatum

Amuria/Uganda

20 (15/5)e

2 (2/0)

4 (4/0)

4 (4/0)

4 (4/0)

 

Butaleja/Uganda

20 (18/2)

0

1 (1/0)

1 (1/0)

1 (1/0)

 

Dokolo/Uganda

20 (12/8)

1 (1/0)

2 (2/0)

1 (1/0)

1 (1/0)

 

Kaberamaido/Uganda

20 (14/6)

0

1 (0/1)

1 (0/1)

1 (0/1)

 

Pallisa/Uganda

20 (10/10)

2 (2/0)

2 (2/0)

2 (2/0)

2 (2/0)

 

Soroti/Uganda

20 (17/3)

2 (2/0)

2 (2/0)

2 (2/0)

2 (2/0)

 

Tororo/Uganda

20 (10/10)

1 (1/0)

1 (1/0)

1 (1/0)

1 (1/0)

 

Subtotal for tick samples

140 (96/44)

8 (8/0)

13 (12/1)

12 (11/1)

12 (11/1)

  1. aPCR was performed using KAPA Blood PCR kit.
  2. bND, not done.
  3. cBlood samples from sheep experimentally infected with E. ruminantium were used as positive controls.
  4. dNA, not applicable.
  5. eTotal no. of ticks (No. of male ticks/No. of female ticks).
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BMC Microbiology

ISSN: 1471-2180

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