Table 2 Inhibitory effects of DNA extracts from field samples on pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP
| Â | Â | No. of samples: | ||||
|---|---|---|---|---|---|---|
Sample type | No. of plasmid copies per reaction | Tested | pCS20 PCR positive | pCS20 real-time PCR positive | pCS20 LAMP positive | sodB LAMP positive |
DNA extracts from bovine blood | 1 | 5 | 0 (0)a | 0 | 0 | 0 |
| Â | 10 | 5 | 0 (0) | 3 | 3 | 3 |
| Â | 102 | 5 | 2 (0) | 5 | 4 | 5 |
| Â | 103 | 5 | 5 (0) | 5 | 5 | 5 |
| Â | 104 | 5 | 5 (5) | 5 | 5 | 5 |
DNA extracts from Amblyomma variegatum | 1 | 5 | 0 (0) | 0 | 0 | 0 |
| Â | 10 | 5 | 0 (0) | 4 | 0 | 0 |
| Â | 102 | 5 | 5 (0) | 5 | 5 | 5 |
| Â | 103 | 5 | 5 (3) | 5 | 5 | 5 |
| Â | 104 | 5 | 5 (5) | 5 | 5 | 5 |