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Table 2 Inhibitory effects of DNA extracts from field samples on pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP

From: Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium

   No. of samples:
Sample type No. of plasmid copies per reaction Tested pCS20 PCR positive pCS20 real-time PCR positive pCS20 LAMP positive sodB LAMP positive
DNA extracts from bovine blood 1 5 0 (0)a 0 0 0
  10 5 0 (0) 3 3 3
  102 5 2 (0) 5 4 5
  103 5 5 (0) 5 5 5
  104 5 5 (5) 5 5 5
DNA extracts from Amblyomma variegatum 1 5 0 (0) 0 0 0
  10 5 0 (0) 4 0 0
  102 5 5 (0) 5 5 5
  103 5 5 (3) 5 5 5
  104 5 5 (5) 5 5 5
  1. aTotal no. of samples positive by using the KAPA Blood PCR kit (Total no. of samples positive by using the AmpliTaq Gold PCR kit).