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Table 1 Comparison of Gliding Rates and Sporulation for mgl mutants

From: Effects of site-directed mutagenesis of mglA on motility and swarming of Myxococcus xanthus

   Gliding on Sporulation
   A-motilitya S-motilityb Percent of WTc
Strain Genotype Average Speed in μm/min (Minutes per reversal)  
WT DK1622 2.6 (20.7) 4.8 (15.6) 100 ± 20
ΔmglBA DK6204 NM 1.9 (10.3) < 0.01
ΔmglBA+mglBA+ MxH2419 2.1 (14.8) 5.3 (10.8) 100 ± 6
ΔmglBA+mglBAG19A MxH2445 NM 2.7 (11.8) < 0.01
ΔmglBA+mglBAG21V MxH2361 NM 2.8 (11.8) 0.01 ± 0.01
ΔmglBA+mglBAL22V MxH2359 1.9 (20.6) 3.8 (12.0) 15 ± 4
ΔmglBA+mglBAK25A MxH2430 NM 2.7 (10.5) < 0.01
ΔmglBA+mglBAT26N MxH2410 NM 1.4 (11.3) < 0.01
ΔmglBA+mglBAD52A MxH2408 NM 1.1 (10.3) < 0.01
ΔmglBA+mglBAT54A MxH2406 NM 2.0 (10.3) < 0.01
ΔmglBA+mglBAT78A MxH2247 0.7 (15.5) 3.0 (11.5) 15 ± 3
ΔmglBA+mglBAT78S MxH2248 1.4 (21.8) 2.7 (7.8) < 0.01
ΔmglBA+mglBAT78D MxH2432 NM NM 0.1 ± 0.0
ΔmglBA+mglBAP80A MxH2357 NM NM 20 ± 6
ΔmglBA+mglBAQ82A MxH2320 NM 2.0 (8.0) < 0.01
ΔmglBA+mglBAQ82R MxH2319 NM 1.8 (10.3) 0.01 ± 0.0
ΔmglBA+mglBAL117/L120A MxH2339 NM 1.4 (9.7) < 0.01
ΔmglBA+mglBAL124K MxH2279 3.6 (8.4) 5.0 (7.6) < 0.01
ΔmglBA+mglBAN141A MxH2338 NM 1.8 (9.8) < 0.01
ΔmglBA+mglBAK142A MxH2365 NM 2.5 (10.2) < 0.01
ΔmglBA+mglBAD144A MxH2367 NM 1.6 (10.6) < 0.01
WT + mglBA+ MxH2375 2.1 (9.7) 8.9 (16.0) 40 ± 10.0
WT + mglB+ MxH2391 2.3 (20.0) 6.6 (15.0) 40 ± 10.0
WT+mglBAG19A MxH2431 1.3 (20.8) 4.0 (19.7) 10 ± 0.6
WT+mglBAG21V MxH2360 2.1 (18.2) 5.2 (15.3) 100 ± 12
WT+mglBAL22V MxH2358 1.8 (15.3) 7.6 (17.5) 2 ± 1.5
WT+mglBAK25A MxH2429 1.8 (21.3) 5.2 (13.6) 60 ± 15
WT+mglBAT26N MxH2409 1.9 (21.0) 8.3 (12.5) < 0.01
WT+mglBAD52A MxH2407 NM 8.7 (12.4) 0.03 ± 0.01
WT+mglBAT54A MxH2405 2.5 (16.2) 9.3 (14.4) 0.01 ± 0.0
WT+mglBAT78A MxH2425 1.7 (25.0) 8.2 (13.4) 30 ± 6
WT+mglBAT78S MxH2426 2.2 (21.4) 7.1 (15.5) < 0.01
WT+mglBAT78D MxH2428 NM 6.0 (12.6) 90 ± 5
WT+mglBAP80A MxH2356 2.0 (23.6) 2.3 (18.3) 40 ± 6
WT+mglBAQ82A MxH2404 1.6 (30.0) 7.5 (13.5) < 0.01
WT+mglBAQ82R MxH2368 2.6 (22.1) 10.0 (22.2) 100 ± 18
WT+mglBAL117/L120A MxH2337 1.3 (15.6) 8.1 (18.4) 100 ± 18
WT+mglBAL124K MxH2278 2.4 (15.1) 3.5 (15.4) < 0.01
WT+mglBAN141A MxH2336 1.7 (NR) 2.1 (17.2) 0.2 ± 0.2
WT+mglBAK142A MxH2364 1.4 (21.3) 9.3 (17.6) 40 ± 6
WT+mglBAD144A MxH2366 1.6 (22.5) 2.4 (11.5) 4 ± 1
  1. Time-lapse microscopy was performed to determine the rates of gliding cells.
  2. a Gliding and reversal rates for cells using A-motility were measured on 1.5% CTPM agarose pads as described in Methods. NM = Cells were nonmotile. NR = no reversals observed.
  3. bGliding and reversal rates for cells using S-motility were measured in 0.5% methylcellulose plus 0.5× CTPM as described in Methods. NM = Cells were nonmotile. Gliding speeds are represented as the average and range of 25 cells from two independent assays.
  4. cSporulation rates are given as a percentage relative to the WT and the standard deviation if available. The ability of MglA mutants to complement the sporulation defects of the ΔmglBA mutant was performed as described in Methods. mgl alleles were introduced into the WT background to determine MglA mutants could interfere with the function of normal MglA during sporulation.