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Table 1 Comparison of Gliding Rates and Sporulation for mgl mutants

From: Effects of site-directed mutagenesis of mglA on motility and swarming of Myxococcus xanthus

  

Gliding on

Sporulation

  

A-motilitya

S-motilityb

Percent of WTc

Strain

Genotype

Average Speed in μm/min (Minutes per reversal)

 

WT

DK1622

2.6 (20.7)

4.8 (15.6)

100 ± 20

ΔmglBA

DK6204

NM

1.9 (10.3)

< 0.01

ΔmglBA+mglBA+

MxH2419

2.1 (14.8)

5.3 (10.8)

100 ± 6

ΔmglBA+mglBAG19A

MxH2445

NM

2.7 (11.8)

< 0.01

ΔmglBA+mglBAG21V

MxH2361

NM

2.8 (11.8)

0.01 ± 0.01

ΔmglBA+mglBAL22V

MxH2359

1.9 (20.6)

3.8 (12.0)

15 ± 4

ΔmglBA+mglBAK25A

MxH2430

NM

2.7 (10.5)

< 0.01

ΔmglBA+mglBAT26N

MxH2410

NM

1.4 (11.3)

< 0.01

ΔmglBA+mglBAD52A

MxH2408

NM

1.1 (10.3)

< 0.01

ΔmglBA+mglBAT54A

MxH2406

NM

2.0 (10.3)

< 0.01

ΔmglBA+mglBAT78A

MxH2247

0.7 (15.5)

3.0 (11.5)

15 ± 3

ΔmglBA+mglBAT78S

MxH2248

1.4 (21.8)

2.7 (7.8)

< 0.01

ΔmglBA+mglBAT78D

MxH2432

NM

NM

0.1 ± 0.0

ΔmglBA+mglBAP80A

MxH2357

NM

NM

20 ± 6

ΔmglBA+mglBAQ82A

MxH2320

NM

2.0 (8.0)

< 0.01

ΔmglBA+mglBAQ82R

MxH2319

NM

1.8 (10.3)

0.01 ± 0.0

ΔmglBA+mglBAL117/L120A

MxH2339

NM

1.4 (9.7)

< 0.01

ΔmglBA+mglBAL124K

MxH2279

3.6 (8.4)

5.0 (7.6)

< 0.01

ΔmglBA+mglBAN141A

MxH2338

NM

1.8 (9.8)

< 0.01

ΔmglBA+mglBAK142A

MxH2365

NM

2.5 (10.2)

< 0.01

ΔmglBA+mglBAD144A

MxH2367

NM

1.6 (10.6)

< 0.01

WT + mglBA+

MxH2375

2.1 (9.7)

8.9 (16.0)

40 ± 10.0

WT + mglB+

MxH2391

2.3 (20.0)

6.6 (15.0)

40 ± 10.0

WT+mglBAG19A

MxH2431

1.3 (20.8)

4.0 (19.7)

10 ± 0.6

WT+mglBAG21V

MxH2360

2.1 (18.2)

5.2 (15.3)

100 ± 12

WT+mglBAL22V

MxH2358

1.8 (15.3)

7.6 (17.5)

2 ± 1.5

WT+mglBAK25A

MxH2429

1.8 (21.3)

5.2 (13.6)

60 ± 15

WT+mglBAT26N

MxH2409

1.9 (21.0)

8.3 (12.5)

< 0.01

WT+mglBAD52A

MxH2407

NM

8.7 (12.4)

0.03 ± 0.01

WT+mglBAT54A

MxH2405

2.5 (16.2)

9.3 (14.4)

0.01 ± 0.0

WT+mglBAT78A

MxH2425

1.7 (25.0)

8.2 (13.4)

30 ± 6

WT+mglBAT78S

MxH2426

2.2 (21.4)

7.1 (15.5)

< 0.01

WT+mglBAT78D

MxH2428

NM

6.0 (12.6)

90 ± 5

WT+mglBAP80A

MxH2356

2.0 (23.6)

2.3 (18.3)

40 ± 6

WT+mglBAQ82A

MxH2404

1.6 (30.0)

7.5 (13.5)

< 0.01

WT+mglBAQ82R

MxH2368

2.6 (22.1)

10.0 (22.2)

100 ± 18

WT+mglBAL117/L120A

MxH2337

1.3 (15.6)

8.1 (18.4)

100 ± 18

WT+mglBAL124K

MxH2278

2.4 (15.1)

3.5 (15.4)

< 0.01

WT+mglBAN141A

MxH2336

1.7 (NR)

2.1 (17.2)

0.2 ± 0.2

WT+mglBAK142A

MxH2364

1.4 (21.3)

9.3 (17.6)

40 ± 6

WT+mglBAD144A

MxH2366

1.6 (22.5)

2.4 (11.5)

4 ± 1

  1. Time-lapse microscopy was performed to determine the rates of gliding cells.
  2. a Gliding and reversal rates for cells using A-motility were measured on 1.5% CTPM agarose pads as described in Methods. NM = Cells were nonmotile. NR = no reversals observed.
  3. bGliding and reversal rates for cells using S-motility were measured in 0.5% methylcellulose plus 0.5× CTPM as described in Methods. NM = Cells were nonmotile. Gliding speeds are represented as the average and range of 25 cells from two independent assays.
  4. cSporulation rates are given as a percentage relative to the WT and the standard deviation if available. The ability of MglA mutants to complement the sporulation defects of the ΔmglBA mutant was performed as described in Methods. mgl alleles were introduced into the WT background to determine MglA mutants could interfere with the function of normal MglA during sporulation.
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BMC Microbiology

ISSN: 1471-2180

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