Figure 3
From: Effects of site-directed mutagenesis of mglA on motility and swarming of Myxococcus xanthus
Immunofluorescence of MglA demonstrates a change in localization in some MglA mutants. Mutant mglA- containing strains were probed with an anti-MglA antibody after fixation as described in Methods. A. WT cells probed with anti-MglA antibody reveal a punctate distribution throughout the cell. B. ΔmglBA strain probed with anti-MglA antibody. No background fluorescence is observed. C. T54A cells probed with anti-MglA antibody. A diffuse fluorescence is observed with no punctate localization. D. L22V cells probed with anti-MglA antibody. Localization similar to that of the WT can be observed.