Reactivity of the polyclonal antibodies anti- Pb SP and deglycosylation assay. A: SDS-PAGE of E. coli extracts. The proteins were stained by Comassie blue. 1: E. coli protein extract; 2: E. coli protein extract obtained after 0.5 mM IPTG treatment. The arrow indicates the protein species corresponding to Pb SP fused to the GST protein. B: Western blot assay, the same extracts as in A reacted to: 1: Mice preimmune serum. 2: Polyclonal antibodies anti-Pb SP. C: SDS-PAGE of P. brasiliensis extracts 1: Total protein extract of yeast cells. 2: Total protein extract of yeast cells treated with endoglycosidase H for 16 h. D: Western blot using the polyclonal antibodies anti-Pb SP reacted with the protein extracts presented in C.