Quantitative real time PCR analysis of gene expression changes after peptide treatment. All the panels show the mean relative expression ± SD (y-axis) of each individual gene upon each peptide treatment as compared to the control treatment with no peptide. (A) and (B) graphs are end-point analyses of expression of the indicated genes (x-axis) after 3 h of peptide treatment; grey bars indicate 5 μM PAF26, black bars 25 μM PAF26, and white bars 5 μM melittin. Note the different expression scales in panels (A) and (B). (C) Graph shows time-course changes of expression of ARG1 following treatment with either 5 μM PAF26 or 5 μM melittin. In all the panels, the genes ALG9, TAF10 and UBC6 were simultaneously used as constitutive references (see Methods for details).