Prevalence of genetic differences in phosphorylcholine expression between nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Table 4 Oligonucleotides used in PCR or for DNA sequence analysis

Gene	Primer sequences^a	Relative position in Rd	Use
licA	F^b: GTAGGATTTGTTAAAACTTGCTACAAGCC	1608693	probe
	R: GGCAATTCCTCTAACAGTTTAAATGCTGCG	1609579
licA	5'F1: GAATAAATTCATAAGAYTCAGAGCCTTAC	1608523	lic1 locus
	5'F2: CAGCTAACCGAGCTTGGGTGAGAAAGTGG	1608476	and
	mid R: GGCGAAACTCATCGAATACGC	1609107	5'-CAAT-
	3'R: GCCCAAAATACAGCGGACAG	1609626	3'
licB	F: ATGCGTGGCTATCTCTTTGGCATAC	1609583	probe
	R: TCATTTTTGTTCCCCTTTGTAATAAAGTG	1610461
licB	5'F: GTTATTTGATATAGCGACGATCATTGAGG	1609316	lic1 locus
	mid F: CGGATTCGCCTTGGCTATTATTTCTTCTTCG	1609957
	mid R: GAGGATATCACTATTTCAGATGACCACCC	1610091
	3'R: GTGTAAATACCCTGTAACAATGACAATATTATCG	1610628
licC	F: ATGAATGCAATCATTTTAGCAGCAGG	1610458	probe
	R: ATGTGGTGATAGTCATCAAGGTTATCC	1611125
licC	mid F: CGTATTGATATTGGTTCACTGAATCAACCC	1610884	lic1 locus
licD	F: ATGAAAAAATTGACTCTCAGAG	1611159	probe
	R: TTACAAAATATACGCTTCTTGAATATG	1611956
licD	5'F: AATTGGGATACCATTCCGATGG	1611016	lic1 locus
	3'R: AAGGGGCGCAAGAGCAGTTAG	1612129	and licD alleles

^a All oligonucleotides based on DNA sequences from H. influenzae strain Rd or from H. haemolyticus lic1 sequence in this paper to make dot-blot hybridization probes or sequence the lic1 locus, the licD gene alleles, or the licA gene tetranucleotide repeats
^b Forward primer begin downstream of licA gene tetranucleotide repeats

ISSN: 1471-2180