Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

Janczarek, Monika; Kutkowska, Jolanta; Piersiak, Tomasz; Skorupska, Anna

doi:10.1186/1471-2180-10-284

Table 4 Bacterial strains, plasmids, and primers used in this study.

From: Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

Strain, plasmid or oligonucleotide primers	Relevant characteristics	Reference
*R. leguminosarum* bv. trifolii 24.2	Wild type, Rif^r, Nx^r	[23]
Rt2440	Rt24.2 derivative carrying rosR with one nucleotide deletion (ΔC177)	[23]
Rt5819	Rt24.2 derivative carrying the mini-Tn5 between 363-364 bp position of pssA	[30]
Rt2472	Rt24.2 derivative carrying the mini-Tn5 between 151-152 bp position of rosR	[30]
Rt2441	Rt24.2 with additional rosR upstream region introduced by pM41 integration, Km^r, Nx^r	This work
*E. coli*
DH5α	supE 44 Δlac U169 (φ80 lacZ Δ M15) hsdR 17 recA 1endA 1gyrA 96 thi-1 relA 1	[67]
S17-1	294 derivative RP4-2Tc::Mu-Km::Tn7 chromosomally integrated	[79]
Plasmids
pK19mobGII	mob, lacZ α, gusA, Km^r	[80]
pBBR1MCS-2	mob, lacZ α, Km^r	[81]
pB31	pUC19 with 1174-bp Bam HI fragment containing Rt24.2 rosR	[23]
pM41	pK19mobGII with 586-bp Eco RI-Pst I fragment from pB31 containing the rosR upstream region	This work
pRC24	pRK7813 with 1174-bp Bam HI fragment containing rosR of Rt24.2	[23]
pBR24	pBBR1MCS-5 with 1174-bp Bam HI fragment containing rosR of Rt24.2	[23]
pEX1	pBBR1MCS-2 with 586-bp Eco RI-Pst I fragment containing the upstream region and the first 60 codons for RosR	This work
pEX8	pBBR1MCS-2 with 372-bp Eco RI-Xba I fragment containing the -403 bp to -32 bp rosR upstream region	This work
pEX9	pBBR1MCS-2 with 219-bp Eco RI-Xba I fragment containing the -403 bp to -185 bp rosR upstream region	This work
pEX60	pBBR1MCS-2 with 278-bp (-96 bp to +182 bp) Eco RI-Pst I fragment containing the first 60 codons for RosR cloned downstream the vector promoter	This work
pBR28	pBBR1MCS-2 with 820-bp (-96 bp to +724 bp) Eco RI-Bam HI fragment containing the full-length rosR cloned downstream the vector promoter	This work
pHC60	Vector with gfp and RK2 stabilization fragment, Tc^r	[39]
Oligonucleotide primers	Sequence (5'-3') ^*
pEP1	ATGCAAGAATTC TGCACAGGAAGC	[23]
pEP5	CGGTCAGGAATTC TAAGAACAGGT	[23]
pEP6	TCGAAACAGGAATTC GATTCCTGC	[23]
pRR1	CGCATTCTAGA CATGTGATCTGCT	[23]
pEP8	AACGGCTCTAGA CTGACACGCCAAA	[23]
pEP9	TCATGCTCTAGA CGATGGCCTCAGT	[23]
rosA	GCGGATCC GCGACTTTACCAGATTTA	[23]
rosB	GTCACGCTCTTCGGAATTC AGGGGT	[23]
rosC	AGGGATCC ATTCTAAACCTGTCGGCA	[23]
rosD	TCGGATCC TGTCGGCAAAGCATAAGA	[23]
rosG1	GACGATCGAATTC GGCCGTCTCTT	This work
rosD4	TTGCGGATCC GCAGATGCCGGT	This work
rosD5	ACCACGCCTGGGATCC AGGAAAA	This work

* Sequences for Eco RI, Bam HI and Xba I restriction sites are underlined.

Back to article page

ISSN: 1471-2180

Contact us

General enquiries: journalsubmissions@springernature.com

BMC Microbiology

Contact us