|
Strain
|
Minimal inhibitory concentration
|
Hyperosmotic
|
Hypo-osmotic
|
|---|
| |
SDS (% w/v)
|
DOC (% w/v)
|
Ethanol (%v/v)
|
stress (%)*
|
stress (%)*
|
|---|
|
Rt24.2
|
0.05 ± 0.005
|
0.10 ± 0.005
|
4.5 ± 0.28
|
77.1
|
51.6
|
|
Rt2440
|
0.02 ± 0.003†
|
0.030 ± 0.003†
|
2.3 ± 0.25†
|
11.5†
|
13.0†
|
|
Rt2441
|
0.02 ± 0.002†
|
0.030 ± 0.003†
|
3.0 ± 0.28
|
11.9†
|
15.2†
|
|
Rt2472
|
0.015 ± 0.002†
|
0.025 ± 0.002†
|
2.6 ± 0.28†
|
10.4†
|
13.3†
|
- * - Strains were grown in TY supplemented with 85 mM NaCl (hyperosmotic) or GYM medium (hypo-osmotic) supplemented with Dilworth's vitamins for 2 days, and the growth was compared with that of strains grown in TY medium. OD600 values were measured. Percentage growth values are the mean and SD from three independent trials.
- † Difference between the wild type and the rosR mutants is statistically significant at P < 0.05 (Student's t test).
