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Table 2 Sensitivity of R. leguminosaru m bv. trifolii ros R mutants to detergents, ethanol, and osmotic stress.

From: Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

Strain Minimal inhibitory concentration Hyperosmotic Hypo-osmotic
  SDS (% w/v) DOC (% w/v) Ethanol (%v/v) stress (%)* stress (%)*
Rt24.2 0.05 ± 0.005 0.10 ± 0.005 4.5 ± 0.28 77.1 51.6
Rt2440 0.02 ± 0.003† 0.030 ± 0.003† 2.3 ± 0.25† 11.5† 13.0†
Rt2441 0.02 ± 0.002† 0.030 ± 0.003† 3.0 ± 0.28 11.9† 15.2†
Rt2472 0.015 ± 0.002† 0.025 ± 0.002† 2.6 ± 0.28† 10.4† 13.3†
  1. * - Strains were grown in TY supplemented with 85 mM NaCl (hyperosmotic) or GYM medium (hypo-osmotic) supplemented with Dilworth's vitamins for 2 days, and the growth was compared with that of strains grown in TY medium. OD600 values were measured. Percentage growth values are the mean and SD from three independent trials.
  2. † Difference between the wild type and the rosR mutants is statistically significant at P < 0.05 (Student's t test).