Figure 7
Developing stages of biofilm formation in R. leguminosarum bv. trifolii wild type 24.2, rosR mutant Rt2472 and Rt2472(pRC24) strains observed after 2 and 4 days. The rosR mutant Rt2472 did not form typical biofilm after 4 days and was restored to the wild type phenotype after introduction of the rosR gene cloned on pRC24 plasmid. Top panel shows 4 dpi biofilms stained with Calcofluor, and the remaining panels show horizontal projected images from 2 and 4 dpi biofilms, with live (Syto-9, green fluorescence) and dead (propidium iodide, red fluorescence) cells. The insets show details of individual stages of biofilm formation.