Figure 3From: Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environmentGel filtration chromatography of exopolysaccharides (EPS) produced by the R. leguminosarum bv. trifolii 24.2 wild type and the rosR mutants (Rt2440 and Rt2441). (A) EPS was fractionated on a Bio-Gel A-5m column, as described in the Methods. The retention times of molecular mass markers: dextran blue (2 MDa), dextran T250 (250 kDa), and dextran T10 (10 kDa) are indicated by arrows. (B) A 500 MHz 1H-NMR spectrometry analysis of the R. leguminosarum wild type and the rosR mutant (Rt2440). (C) The glycosyl components and non-carbohydrate substituents of EPS from the wild type and the mutant Rt2440. (D) Silver-stained Tricine SDS-PAGE profiles of LPS from the wild type and the rosR mutants. LPSs (2 μg) were loaded in 2 μl sample buffer. Lanes: 1- Salmonella enterica sv. Typhimurium (Sigma), 2- wild type Rt24.2, 3- Rt2440, 4- Rt2441. LPS I, high-molecular-weight LPS; LPS II, low-molecular-weight LPS. (E) The glycosyl composition of polysaccharides lacking lipid A released from LPS by mild acid hydrolysis from the wild type and the rosR mutant (Rt2440).Back to article page