Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogenous expression

BMC Microbiology

Table 3 Cells expressing SOS regulated genes in the wild type RW118

*gfp* transcriptional fusion	% of intensely fluorescent cells	Fluorescence threshold level*	Cell count	HI Distal	Proximal^†
caa-gfp (pSC300)	0.62	41	15555	11.52	9.73
cna-gfp (pSC301)	0.51	41	9793	7.55	11.61
ce1a-gfp (pSC302)	0.48	41	12197	7.48	11.06
ce7a-gfp (pSC303)	1.55	41	9338	12.44	12.98
recA-gfp (pSC201)	3.1	57	8366	4.31
lexA-gfp (pSC200)	1.48	57	5089	6.39	8.31
umuDC-gfp (pSC202)	0.09	31	2083	2.77

*Fluorescence threshold level is defined as the point of clear transition from basal level (large majority of cells) to high fluorescence intensity.
^† Designated with regard to the ATG codon.

ISSN: 1471-2180