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Table 2 Characteristics of the fluorescent dyes used in confocal laser scanning microscopy

From: A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

Fluorescent substance Manufacturer Excitation wavelength (Laser) in [nm] Emission range in [nm] Concentration/incubation time/temperature Fluorescence of
Acridine orange Acridine orange - zinc chloride, Applichem GmbH, Darmstadt; Germany Argon 458 505-550 BP 592-753 BP 200 μg/mL;
2-5 min; RT
nucleic acids
DAPI Dapi Biochemica, Applichem GmbH, Darmstadt; Germany Diode 405 420-480 BP 20 μg/mL;
30 min; RT
nucleic acids
ConA-Alexa Fluor 488 Concanavalin A - Alexa Fluor® 488 conjugated, Invitrogen Molecular Probes, Eugene, USA Argon 488 505-530 BP 10 μg/mL;
30 min; RT
CTC CTC (5-Cyano-2,3-di-4-tolyl-tetraolium chloride), Polysciences Inc.; Warrington, USA Diode 561 575 LP 1.25 mg/mL;
3 h; RT
redox activity