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Table 1 All bacterial strains, plasmids used and constructed.

From: Identification of tomato plant as a novel host model for Burkholderia pseudomallei

Name Description Source or Reference
pK18mobsac B oriT; KmR; sacB gene [32]
pGEM-tet pGEM containing a tetracycline resistance cassette, TetR, AmpR Y. Chen, unpublished
pCLOXZ1 pGEM containing a zeocin resistance cassette, ZeoR, AmpR Y. Chen, unpublished
pT3SS1/upstream/downstream/tet pK18mobsac B containing upstream and downstream of TTSS1 flanking a tet cassette, KmR, TetR This study
pT3SS2/upstream/downstream/tet pK18mobsac B containing upstream and downstream of TTSS2 flanking a tet cassette, KmR, TetR This study
pT3SS3/upstream/downstream/zeo pK18mobsac B containing upstream and downstream of TTSS3 flanking a zeo cassette, KmR, ZeoR This study
E. coli   
   DH5α Infection strain Lab stock
   TG1 Cloning host Zymo Research
   SM10λpir Conjugation strain [33]
B. thailandensis   
   ATCC700388   ATCC
B. pseudomallei   
   K96243 Clinical isolate Thailand
   561 Kangaroo isolate Eu Hian Yap, unpublished
   612, 490 Avian isolates Eu Hian Yap, unpublished
   77/96, 109/96 Soil isolates Eu Hian Yap, unpublished
   KHW Wild-type parental strain, clinical isolate, KmS [20]
   KHWΔT3SS1 BPSS1386-1411 region was replaced with tet cassette, TetR, KmS This study
   KHWΔT3SS2 BPSS1592-1629 region was replaced with tet cassette, TetR, KmS This study
   KHWΔT3SS3 BPSS1520-1552 region was replaced with zeo cassette, ZeoR, KmS This study