Table 2 Oligonucleotides used in the present study
From: A rapid and simple method for constructing stable mutants of Acinetobacter baumannii
Oligonucleotide name | Sequence (5' to 3') | Application |
|---|---|---|
33intUP | CTGGTGACGTTGCTGGTACA | Construction of the omp33::TOPO mutant |
33intDW | CGTTACCGATGATACCGAAG | Construction of the omp33::TOPO mutant |
33extUP | CCTTAACATTACGTTTCATC | Confirmation of the omp33::TOPO mutant |
33extDW | CATGTAAGATGCACCAACTGC | Confirmation of the omp33::TOPO mutant |
Kmup | CCGGAATTGCCAGCTGGG | Kanamycin amplification |
Kmdw | TTCAGAAGAACTCGTCAAG | Kanamycin amplification |
33upFW | GCTGAGCTCGTAAAGTCTGATG | Construction and confirmation of the Δomp33::Km mutant |
33upintRV | CCCAGCTGGCAATTCCGGGGCTAATAATACAGCAGTGG | Construction of the Δomp33::Km mutant |
33dwintFW | CTTGACGAGTTCTTCTGAAGGCTTAAATGCTAAATTCCG | Construction of the Δomp33::Km mutant |
33dwRV | CGTTGCCTTTTACCGTAGTC | Construction and confirmation of the Δomp33::Km mutant |
33FWnest | GCAATTGAATTGTGTGAC | Construction of the Δomp33::Km mutant |
33RVnest | TGATAGCAATTCAAGAGG | Construction of the Δomp33::Km mutant |
OxyupFW | AGTTAAAAAAAATTGAAGAAA | Construction and confirmation of the ΔoxyR::Km mutant |
OxyupintRV | CCCAGCTGGCAATTCCGGTCACTTGATGATCTCGATTTA | Construction of the ΔoxyR::Km mutant |
OxydwintFW | CTTGACGAGTTCTTCTGAATGAAGATCACCAGTTAATGG | Construction of the ΔoxyR::Km mutant |
OxydwRV | TATATTAACCATATTGAAGCC | Construction and confirmation of the ΔoxyR::Km mutant |
OxyFWnest | GCAACTTGATGCAGCGGT | Construction of the ΔoxyR::Km mutant |
OxyRVnest | TCAACGTAGCTACTATCC | Construction of the ΔoxyR::Km mutant |
SoxupFW | ATGAAAGAAAAAAACTATATA | Construction and confirmation of the ΔsoxS::Km mutant |
SoxupintRV | CCCAGCTGGCAATTCCGGATATTTACTTAGGGCTTGTTT | Construction of the ΔsoxS::Km mutant |
SoxdwintFW | CTTGACGAGTTCTTCTGAAGAACAATGTCCATT AGAAA | Construction of the ΔsoxS::Km mutant |
SoxdwRV | TCTGACTTCGTTTTTTGCTTA | Construction and confirmation of the ΔsoxS::Km mutant |
SoxFWnest | AATTGCACGTTGCGATAG | Construction of the ΔsoxS::Km mutant |
SoxRVnest | TAAACCAGATAGCCCAAC | Construction of the ΔsoxS::Km mutant |
ATG33XbaI* | ATCGTCTAGACCCAGCTTTATCTCTTGTTA | Cloning of the omp33 gene |
STOP33NcoI* | ATCGCCATGGGGACTGGACTCAGGAAGATTTG | Cloning of the omp33 gene |
SoxRTup | AGGAACTGTAATTGCACG | RT-PCR for soxR detection |
SoxRTrv | CCAATCGAGAGATAGCTC | RT-PCR for soxR detection |
OxyRTup | ATGGCTGCATTACCCTCACT | RT-PCR for oxyR detection |
OxyRTrv | GCGCTCTACAATCTTCTCAC | RT-PCR for oxyR detection |
GyrBup | ATGAGTTCAGAGTCTCAATCA | RT-PCR for gyrB detection |
GyrBrv | CTGTTAAACCTTCACGCGCAT | RT-PCR for gyrB detection |
T7 | AATACGACTCACTATAGGG | Universal primer of the pCR-BluntII-TOPO plasmid |
SP6 | ATTTAGGTGACACTATAG | Universal primer of the pCR-BluntII-TOPO plasmid |
pETRAFW | TTCTTCGTGAAATAGTGATGATTTTT | Primer of the pET-RA plasmid |
pETRARV | CTGTTTCATATGATCTGGGTATC | Primer of the pET-RA plasmid |