RT-qPCR analysis of different S . Typhimurium luxS mutants with MicA primers. MicA sRNA expression levels were measured with RT-qPCR as described in the Methods section. Representative means and standard deviations of three RT-qPCRs are shown. Gene expression is expressed relative to the wildtype SL1344 level. CMPG5602: SL1344 ΔluxS deletion mutant; CMPG5702: SL1344 luxS::KmR insertion mutant; CMPG5630: SL1344 ΔluxS2 deletion mutant.