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Table 4 Quantitative RT-PCR analysis on H-NS targets involved in acid stress resistance

From: Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli

  Expression ratio
Gene hns/wild-type hns gadE /wild-type hns rcsB /wild-type hns hdfR /wild-type hns adiY /wild-type
Glutamate-dependent specific pathway
gadA 1 137.21 nd Nd 150.93 41.31
dctR 1 34.66 nd Nd 34.32 8.84
yhiM 10.75 3.41 3.40 10.90 11.36
aslB 12.92 0.66 1.10 0.69 1.32
gltD 1 1.68 nd Nd 0.48 0.52
Arginine-dependent specific pathway
adiA 16.89 nd Nd nd 0.70
adiC 11.62 nd Nd nd 1.41
Lysine-dependent specific pathway
cadC 4.62 5.77 6.38 nd nd
General acid stress resistance pathway
hdeA 1 32.37 nd Nd 41.20 6.55
hdeD 18.96 nd Nd 17.57 5.89
adiY 5.08 5.00 5.00 nd nd
  1. nd: non-determined.
  2. 1: Since several genes are organized in operon and/or are highly homologous to each other, results obtained with gadA also corresponds to gadBC; with gltD to gltB; with hdeA to hdeB; with dctR to slp.
  3. Quantitative RT-PCR were performed on total RNA isolated from exponential growth phase cultures. Standard deviations were less than 20% of the mean.