Transcription analysis of mycobacterial rhomboids. A. RT-PCR amplification of Rv0110 cDNA from MTC and M. smegmatis mRNA. Lanes: M, 1 kbp DNA ladder; 1, M. tuberculosis H37Rv; 2, M. tuberculosis BN44; 3, M. bovis BCG; 4, M. bovis JN55 and 5, M. smegmatis SMR5. B. RT-PCR amplification of Rv1337 cDNA from MTC, MAC and M. smegmatis mRNA. Lanes: L, 100 bp DNA ladder; 1, M. tuberculosis H37Rv; 2, M. tuberculosis BN44; 3, M. bovis BCG; 4, M. bovis JN55; 5, M. avium; 6, M. avium subsp. Paratuberculosis; 7, M. smegmatis SMR5; 8, negative control (M. tuberculosis mRNA, not reverse transcribed); 9, negative control (E. coli mRNA, reverse transcribed); 10, negative control (water). C: Similar assays as in B showing cDNA amplification (~350 bp) of the internal fragment of Rv1337 othologs. Negative controls for panel "A" (not shown) were similar to 8, 9 & 10.