Table 1 Oligonucleotides used in this work.
From: A bacteria-specific 2[4Fe-4S] ferredoxin is essential in Pseudomonas aeruginosa
name | Sequence (5' - 3') | comments |
|---|---|---|
FDX-Eco | GAATTC GACCATGTGATCATCGCG | Construction of lacZ reporter |
FDX-Bam | GGATCC TCAATCGTCAGTGATTTTCAGGG | Construction of lacZ reporter |
FDX-Eco200 | GAATTCgccgctctcggcggg | Construction of lacZ reporter |
FDX-F1 | GGGAAGGCGAATGTCTTCCTC | Deletion of fdx1 |
FDX-R1 | GGATCAGGCCTTGCCCTC GAG GGACATCTAACAACTCC | Deletion of fdx1 |
FDX-F2 | CTCGAG GGCAAGGCCTGATCCCC | Deletion of fdx1 |
FDX-R2 | GGGAGTCCCGAGCCGTAAGG | Deletion of fdx1 |
FDX-F3 | CCCGGG TTGTTAGTA GTCCCTGAAAATC | Insertion mutagenesis of fdx1 (inverted nucleotides in bold) |
FDX-R4 | CCCGGG CACTGCGGCTCGTCGTAG | Insertion mutagenesis of fdx1 |
FDX-F0 | CTGGCGCACTTCGTCAAGGAG | Amplification of the genomic copy of fdx1 |
FDX-R0 | GCAGAAGGAAGAATCCACCGC | Amplification of the genomic copy of fdx1 |
FDX-PstI | CCCTGCAG GTCGCGGTTGGAGTTGTTAG | Construction of pJN-Fdx1 |
FDX-XbaI | GGTCTAGA AGGACAGGCGCCGGCGG | Construction of pJN-Fdx1 |
F2PaFd | CGCAAGGCGAAGAAATCTAT | Primer for fdx1 RT-PCR |
R2PaFd | TCCTTGCTCTCGACATTGG | Primer for fdx1 RT-PCR |
R1bacRNA | AGAGTTTGATCCTGGCTCAG | Primer for rRNA RT-PCR |
R2bacRNA | ACTGCTGCCTCCCGTAGGAG | Primer for rRNA RT-PCR |