Skip to main content

Table 3 Transcript and protein expression in cattle MAP under iron-replete (HI) conditions

From: Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

  MAP ORF ID Predicted function aFold change
    Protein Transcript
Metabolism
  MAP0150c FadE25_2 (acyl-coA dehydrogenase) 1.72 ± 0.1 1.88 ± 0.2
  MAP0789 acetyl-CoA acetyltransferase 1.73 ± 0.3 1.56 ± 0.1
  MAP1846c ATP phosphoribosyltransferase 1.69 ± 0.2 3.68 ± 0.3
  MAP2332c Fas (fatty acid synthase) 1.61 ± 0.5 2.28 ± 0.4
  MAP3404 AccA3 (acetyl-/propionyl-coenzyme A) 1.45 ± 0.1 2.18 ± 0.2
  MAP3698c succinate dehydrogenase 1.89 ± 0.3 4.57 ± 0.5
Cellular processes
  MAP1339 iron regulated conserved protein 1.62 ± 0.2 0.78 ± 0.3
  MAP1653 thiol peroxidase 1.79 ± 0.5 2.29 ± 0.2
Information storage and processing
  MAP2907c translation initiation factor IF-2 1.57 ± 0.2 1.89 ± 0.2
  MAP2945c ribosome releasing factor 1.66 ± 0.3 2.11 ± 0.5
  MAP4113 50S ribosomal protein L1 1.61 ± 0.1 1.57 ± 0.2
  MAP4125 rplJ 50S ribosomal protein L10 1.52 ± 0.1 1.66 ± 0.5
  MAP4142 fusA elongation factor G 2.13 ± 0.4 3.05 ± 0.3
  MAP4160 rpsJ 30S ribosomal protein S10 1.68 ± 0.3 2.87 ± 0.4
  MAP4181 rpsH 30S ribosomal protein S8 1.79 ± 0.5 2.42 ± 0.1
  MAP4233 rpoA DNA-directed RNA polymerase 1.56 ± 0.1 1.65 ± 0.4
Poorly characterized pathways
  MAP0216 FbpA antigen 85-A 1.87 ± 0.2 2.16 ± 0.3
  MAP1122 mycobacterial integration host factor 1.73 ± 0.3 2.00 ± 0.5
  1. a MAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of cattle MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log2 ratio of HI/LI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in HI compared to LI. Genes are annotated based on the motif searches in KEGG database.