Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

BMC Microbiology

Table 2 Transcript and protein expression in sheep MAP under iron-limiting (LI) conditions

	MAP ORF ID	Predicted function	^aFold change
			Protein	Transcript
Metabolism
	MAP3564	methyltransferase	1.54 ± 0.1	1.58 ± 0.6
	MAP1942c	CbhK, ribokinase	1.74 ± 0.3	2.05 ± 1.0
	MAP2286c	thioredoxin domain containing protein	1.82 ± 0.1	2.04 ± 0.3
	MAP1997	acyl carrier protein	1.90 ± 0.5	1.68 ± 0.5
Cellular processes
	MAP4340	TrxC, thioredoxin	1.50 ± 0.4	2.29 ± 0.3
	MAP3840	DnaK molecular chaperone	1.63 ± 0.6	3.52 ± 0.5
Information storage and processing
	MAP4142	FusA, elongation factor G	1.52 ± 0.2	2.58 ± 0.7
	MAP4268c	transcriptional regulatory protein	1.52 ± 0.3	1.50 ± 0.1
	MAP4233	DNA-directed RNA polymerase alpha subunit	1.56 ± 0.1	1.83 ± 0.3
	MAP3024c	DNA binding protein, HU	1.60 ± 0.6	1.81 ± 0.5
	MAP4184	30S ribosomal protein S5	1.75 ± 0.1	1.55 ± 0.3
	MAP3389c	response regulator	1.94 ± 0.3	1.59 ± 0.2
	MAP4111	transcription antitermination protein, NusG	1.98 ± 0.3	1.82 ± 0.5
	MAP4143	elongation factor Tu	2.08 ± 0.4	2.16 ± 0.1
Poorly characterized pathways
	MAP2844	conserved alanine and arginine rich protein	1.54 ± 0.2	2.27 ± 0.5
	MAP3433	initiation of DNA replication	1.63 ± 0.1	1.91 ± 0.2
	MAP0126	transcriptional regulator like protein	1.75 ± 0.6	1.50 ± 0.2
	MAP1065	pyridox oxidase	1.83 ± 1.0	1.52 ± 0.5

^aMAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of sheep MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log₂ ratio of LI/HI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in LI compared to HI. Genes are annotated based on the motif searches in KEGG database.

ISSN: 1471-2180