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Table 2 Transcript and protein expression in sheep MAP under iron-limiting (LI) conditions

From: Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

  MAP ORF ID Predicted function aFold change
    Protein Transcript
Metabolism
  MAP3564 methyltransferase 1.54 ± 0.1 1.58 ± 0.6
  MAP1942c CbhK, ribokinase 1.74 ± 0.3 2.05 ± 1.0
  MAP2286c thioredoxin domain containing protein 1.82 ± 0.1 2.04 ± 0.3
  MAP1997 acyl carrier protein 1.90 ± 0.5 1.68 ± 0.5
Cellular processes
  MAP4340 TrxC, thioredoxin 1.50 ± 0.4 2.29 ± 0.3
  MAP3840 DnaK molecular chaperone 1.63 ± 0.6 3.52 ± 0.5
Information storage and processing
  MAP4142 FusA, elongation factor G 1.52 ± 0.2 2.58 ± 0.7
  MAP4268c transcriptional regulatory protein 1.52 ± 0.3 1.50 ± 0.1
  MAP4233 DNA-directed RNA polymerase alpha subunit 1.56 ± 0.1 1.83 ± 0.3
  MAP3024c DNA binding protein, HU 1.60 ± 0.6 1.81 ± 0.5
  MAP4184 30S ribosomal protein S5 1.75 ± 0.1 1.55 ± 0.3
  MAP3389c response regulator 1.94 ± 0.3 1.59 ± 0.2
  MAP4111 transcription antitermination protein, NusG 1.98 ± 0.3 1.82 ± 0.5
  MAP4143 elongation factor Tu 2.08 ± 0.4 2.16 ± 0.1
Poorly characterized pathways    
  MAP2844 conserved alanine and arginine rich protein 1.54 ± 0.2 2.27 ± 0.5
  MAP3433 initiation of DNA replication 1.63 ± 0.1 1.91 ± 0.2
  MAP0126 transcriptional regulator like protein 1.75 ± 0.6 1.50 ± 0.2
  MAP1065 pyridox oxidase 1.83 ± 1.0 1.52 ± 0.5
  1. aMAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of sheep MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log2 ratio of LI/HI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in LI compared to HI. Genes are annotated based on the motif searches in KEGG database.