Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

BMC Microbiology

Table 1 Transcript and protein expression in cattle MAP under iron-limiting (LI) conditions

	MAP ORF ID	Predicted function	^aFold change
			Protein	Transcript
Metabolism
	MAP1587c	alpha amylase	2.03 ± 0.2	2.87 ± 0.7
	MAP1554c	FadE33_2 (acyl-coA synthase)	1.79 ± 0.5	1.88 ± 0.8
	MAP2307c	pdhC alpha-keto acid dehydrogenase	1.68 ± 0.3	2.52 ± 0.4
	MAP3189	FadE23 (acyl-CoA dehydrogenase)	2.41 ± 0.2	3.51 ± 1.0
	MAP3694c	FadE5 (acyl-CoA dehydrogenase)	1.87 ± 0.8	3.15 ± 0.2
Cellular processes
	MAP3701c	heat shock protein	2.18 ± 0.6	2.48 ± 0.3
	MAP1188	FeS assembly protein SufD	2.23 ± 1.0	2.73 ± 0.2
	MAP1189	FeS assembly ATPase SufC	1.78 ± 0.5	2.03 ± 0.1
	MAP4059	heat shock protein HtpX	1.48 ± 0.1	1.66 ± 0.5
Poorly characterized pathways
	MAP1012c	patatin-like phospholipase	1.67 ± 0.3	1.56 ± 0.3
	MAP1944c	iron suphur cluster biosynthesis	1.56 ± 0.9	1.66 ± 0.2
	MAP2482	Glyoxalase/Bleomycin resistance	1.84 ± 0.3	2.19 ± 0.8
	MAP3838c	RES domain containing protein	1.50 ± 0.7	2.40 ± 0.2

^aMAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of cattle MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log₂ ratio of LI/HI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in LI compared to HI. Genes are annotated based on the motif searches in KEGG database.

ISSN: 1471-2180