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Table 1 Transcript and protein expression in cattle MAP under iron-limiting (LI) conditions

From: Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

  MAP ORF ID Predicted function aFold change
    Protein Transcript
Metabolism     
  MAP1587c alpha amylase 2.03 ± 0.2 2.87 ± 0.7
  MAP1554c FadE33_2 (acyl-coA synthase) 1.79 ± 0.5 1.88 ± 0.8
  MAP2307c pdhC alpha-keto acid dehydrogenase 1.68 ± 0.3 2.52 ± 0.4
  MAP3189 FadE23 (acyl-CoA dehydrogenase) 2.41 ± 0.2 3.51 ± 1.0
  MAP3694c FadE5 (acyl-CoA dehydrogenase) 1.87 ± 0.8 3.15 ± 0.2
Cellular processes     
  MAP3701c heat shock protein 2.18 ± 0.6 2.48 ± 0.3
  MAP1188 FeS assembly protein SufD 2.23 ± 1.0 2.73 ± 0.2
  MAP1189 FeS assembly ATPase SufC 1.78 ± 0.5 2.03 ± 0.1
  MAP4059 heat shock protein HtpX 1.48 ± 0.1 1.66 ± 0.5
Poorly characterized pathways     
  MAP1012c patatin-like phospholipase 1.67 ± 0.3 1.56 ± 0.3
  MAP1944c iron suphur cluster biosynthesis 1.56 ± 0.9 1.66 ± 0.2
  MAP2482 Glyoxalase/Bleomycin resistance 1.84 ± 0.3 2.19 ± 0.8
  MAP3838c RES domain containing protein 1.50 ± 0.7 2.40 ± 0.2
  1. aMAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of cattle MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log2 ratio of LI/HI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in LI compared to HI. Genes are annotated based on the motif searches in KEGG database.