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Table 5 Detection limits of BoNT DNA in crude toxin supernatants

From: Universal and specific quantitative detection of botulinum neurotoxin genes

  Bot A Bot B Bot E Bot F
Crude Toxin 2 ng LOD    
Crude Toxin 200 pg   LOD   LOD
Crude Toxin 20 pg    0.8 (LOD)  
Crude Toxin 2 pg     
Crude Toxin 200 fg   11.7   2.58
Crude Toxin 20 fg 29.2    
  1. LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.
  2. Indicated in this table is the sensitivity of quantitative PCR for type-specific BoNT detection. Crude toxin supernatants with equivalent toxin protein amounts are listed. Values included in this table are the exact copy number of BoNT DNA detected in crude toxin preparations at the indicated amounts of protein. LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.