Table 4 Cross reactivity and specificity of primers and probes with all subtypes of C. botulinum Subtype Specific Primer and Probe set Used in Real Time PCR
From: Universal and specific quantitative detection of botulinum neurotoxin genes
BoNT subtype | strain | A | B | C | D | E | F | G |
|---|---|---|---|---|---|---|---|---|
A1 | Hall | + | - | - | - | - | - | - |
A1 | CDC 1757 | + | - | - | - | - | - | - |
A1 | CDC 1744 | + | - | - | - | - | - | - |
A2 | Kyoto-F | + | - | - | - | - | - | - |
A2b | CDC 1436 | + | + | - | - | - | - | - |
A3 | Loch Maree | + | - | - | - | - | - | - |
B1 | Okra | - | + | - | - | - | - | - |
B1 | CDC 1656 | - | + | - | - | - | - | - |
B1 | CDC 1758 | - | + | - | - | - | - | - |
B2 | 213B | - | + | - | - | - | - | - |
B2 | CDC 1828 | - | + | - | - | - | - | - |
B3 | CDC 795 | - | + | - | - | - | - | - |
B4 (npB) | Eklund 17B | - | + | - | - | - | - | - |
Ba4 | CDC 657 | + | + | - | - | - | - | - |
Bf | An436 | - | + | - | - | - | - | - |
C | Stockholm | - | - | + | - | - | - | - |
C/D | 6813 | - | - | + | - | - | - | - |
D | ATCC 11873 | - | - | - | + | - | - | - |
D | 1873 | - | - | - | + | - | - | - |
D/C | VPI 5995 | - | - | - | + | - | - | - |
E1 | Beluga | - | - | - | - | + | - | - |
E2 | CDC 5247 | - | - | - | - | + | - | - |
E2 | CDC 5906 | - | - | - | - | + | - | - |
E3 | Alaska E43 | - | - | - | - | + | - | - |
E4 (It butyr) | BL5262 | - | - | - | - | + | - | - |
F1 (prot) | Langeland | - | - | - | - | - | + | - |
F2 (np) | Eklund 202F | - | - | - | - | - | + | - |
F3 (baratii) | Orange | - | - | - | - | - | + | - |
G | 1354 | - | - | - | - | - | - | + |