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Table 4 Cross reactivity and specificity of primers and probes with all subtypes of C. botulinum Subtype Specific Primer and Probe set Used in Real Time PCR

From: Universal and specific quantitative detection of botulinum neurotoxin genes

BoNT subtype strain A B C D E F G
A1 Hall + - - - - - -
A1 CDC 1757 + - - - - - -
A1 CDC 1744 + - - - - - -
A2 Kyoto-F + - - - - - -
A2b CDC 1436 + + - - - - -
A3 Loch Maree + - - - - - -
B1 Okra - + - - - - -
B1 CDC 1656 - + - - - - -
B1 CDC 1758 - + - - - - -
B2 213B - + - - - - -
B2 CDC 1828 - + - - - - -
B3 CDC 795 - + - - - - -
B4 (npB) Eklund 17B - + - - - - -
Ba4 CDC 657 + + - - - - -
Bf An436 - + - - - - -
C Stockholm - - + - - - -
C/D 6813 - - + - - - -
D ATCC 11873 - - - + - - -
D 1873 - - - + - - -
D/C VPI 5995 - - - + - - -
E1 Beluga - - - - + - -
E2 CDC 5247 - - - - + - -
E2 CDC 5906 - - - - + - -
E3 Alaska E43 - - - - + - -
E4 (It butyr) BL5262 - - - - + - -
F1 (prot) Langeland - - - - - + -
F2 (np) Eklund 202F - - - - - + -
F3 (baratii) Orange - - - - - + -
G 1354 - - - - - - +
  1. "+/-" indicates amplification/no amplification by real time PCR
  2. Indicated in the table are all strains tested with each type-specific BoNT primer and probe set. A (+/-) indicates amplification/no amplification by real time PCR.