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Table 1 NTNH gene detection on C. botulinum and other clostridial strains

From: Universal and specific quantitative detection of botulinum neurotoxin genes

BoNT subtype strain PCR(DNA)a (culture supernatant)b other clostridia strain PCR(DNA)a
A1 Hall + + C. absonum ATCC 27555 -
A1 CDC 1757 + + C. baratii e ATCC 27638 -
A1 CDC 1744 + + C. bifermentans ATCC 638 -
A2 Kyoto-F + + C. haemolyticum ATCC 9650 -
A2b CDC 1436 + + C. hastiforme ATCC 25772 -
A3 Loch Maree + + C. histolyticum ATCC 19401 -
B1 Okra + + C. novyi ATCC 17861 -
B1 CDC 1656 + + C. novyi ATCC 19402 -
B1 CDC 1758 + + C. novyi A ATCC 19402 -
B2 213B + + C. novyi B ATCC 2706 -
B2 CDC 1828 + + C. perfringens A ATCC 3624 -
B4 (npB) Eklund 17B + + C. perfringens A ATCC 12915 -
Ba4 CDC 657 + + C. perfringens A ATCC 12917 -
Bf An436 + + C. perfringens A ATCC 12918 -
C Stockholm + - C. perfringens A ATCC 12919 -
C/D 6813 + - C. perfringens A ATCC 13124 -
D ATCC 11873 + + C. perfringens B ATCC 3626 -
D 1873 + nd C. perfringens D ATCC 3629 -
D/C VPI 5995 + + C. perfringens D ATCC 3630 -
E1 Beluga + - C. perfringens D ATCC 3631 -
E2 CDC 5247 + nt C. perfringens D ATCC 12920 -
E2 CDC 5906 + nt C. perfringens E ATCC 27324 -
E3 Alaska E43 + + C. ramosum ATCC 25582 -
E4 (It butyr)c BL5262 + - C. septicum ATCC 12464 -
F1 (prot) Langeland + + C. sordelli ATCC 9714 -
F2 (np) Eklund 202F + - C. sporogenes ATCC 19404 -
F3 (baratii)d Orange + nt C. sporogenes ATCC3854 -
G 1354 + nd C. subterminale ATCC 25774 -
     C. tertium ATCC 14573 -
     C. tetani ATCC 10799 -
     C. tetani ATCC19406 -
  1. a +/- indicates presence/absence of 101 bp band on agarose gel. Samples are purified DNA from bacterial cultures as described in the Methods section.
  2. b Samples originate from filtered culture supernatants containing crude toxin. +/- indicates presence/absence of 101 bp band on agarose gel. nd = not detected, nt = not tested.
  3. c BoNT E-producing strain of C. butyricum isolated from an infant case in Italy.
  4. d BoNT F-producing strain of C. baratii.
  5. eNon-toxin producing strain of C, baratii.
  6. Results from conventional PCR detection of NTNH. A (+/-) indicates presence/absence of 101 bp band by agarose gel, respectively. DNA results indicate PCR detection of NTNH in purified DNA from both C botulinum and other Clostridial strains. Culture supernatant results indicate amplification of DNA within crude culture supernatants. NT indicates samples that were not tested.