Figure 4
From: The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
Verification of the expression of small RNA RyhB by RT-PCR. L: DNA ladder; 1. PCR amplification of S. oneidensis RNA without reverse transcription; 2. PCR amplification of sample after reverse transcription of RNA. The presence of the ~119 bp PCR product validates the expression of RyhB RNA. 3 and 4: PCR on two control intergenic regions (Chr. 367734-367820 and 796545-796665). The absence of PCR products indicates that genomic DNA has been completely removed from the RNA templates used for RT-PCR.