Figure 2

Treatment of OmpA-sal decreases endocytic capacities of DCs and induces production of IL-12. On day 6, the cells were cultured at standard conditions for another 24 h in the presence of 200 ng/ml of LPS or 100, 200, and 400 ng/ml of OmpA-sal and harvested, and stained with a PE-conjugated anti-CD11c⁺ antibody. Endocytic capacity at 37°C or 4°C was assessed by dextran-FITC uptake (A). The percentage of positive cells is indicated for each condition and is representative of the data of three separate experiments (B). Analysis of IL-12p70 and IL-10 cytokine production in magnetic bead-purified DCs by ELISA (C). The data are the means and standard deviation of three experiments. *p < 0.05, **p < 0.01 vs. untreated DCs.