Figure 1

OmpA-sal is not cytotoxic and induces the expression of co-stimulatory molecules in DCs. BM-DCs were cultured for 24 h in the presence of 200 ng/ml of LPS or 100, 200, 400, and 800 ng/ml of OmpA-sal and analyzed by flow cytometry. The DCs were stained with annexin V and PI. The percentage of positive cells is indicated (A). The cells were gated to exclude CD11c⁺ cells. Medium, untreated control; LPS, positive control. DCs were stained with anti-CD80, anti-CD86, anti-MHC class I, and anti-MHC class II molecules (B). The data are representative of three experiments that yielded similar results.