T-RFPred: a nucleotide sequence size prediction tool for microbial community description based on terminal-restriction fragment length polymorphism chromatograms

Table 2 Phylogenetic information for the 16S rRNA sequences present in the 4926 and GOS datasets that matched selected chromatogram peaks shown in Figure 2

Dataset	Peak	Chromatograms	Number of sequences	Taxonomic group
4926	1	Cfo I, Hae III	243	Total
			146	Roseobacter
			53	Gammaproteobacteria
			36	Alphaproteobacteria
			4	Planctomycetes
			2	Betaproteobacteria
			1	Cyanobacteria
			1	Firmicutes
4926	1	Cfo I, Hae III, Alu I	124	Total
			111	Roseobacter
			7	Alphaproteobacteria
			4	Gammaproteobacteria
			2	Betaproteobacteria
4926	2	Cfo I, Hae III	207	Total
			152	SAR11
			51	Firmicutes
			1	Alphaproteobacteria
			1	Unclassified Bacteria
4926	2	Cfo I, Hae III, Alu I	148	Total
			119	SAR11
			29	Firmicutes
GOS	1	Cfo I, Hae III	263	Total
			231	Roseobacter
			18	Alphaproteobacteria
			13	Gammaproteobacteria
			1	Actinobacteria
GOS	1	Cfo I, Hae III, Alu I	243	Total
			229	Roseobacter
			12	Alphaproteobacteria
			1	Gammaproteobacteria
			1	Actinobacteria
GOS	2	Cfo I, Hae III	560	Total
			559	SAR11
			1	Alphaproteobacteria
GOS	2	Cfo I, Hae III, Alu I	269	Total
			269	SAR11

Sequences that matched the fragment sizes were analyzed using 2-3 different restriction enzymes as indicated. Alphaproteobaceria sensu latu refers to any bacterial sequences in the class that were not either Roseobacter or SAR11. See Experimental Procedures in the Additional File 1 for details.

ISSN: 1471-2180