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Figure 5 | BMC Microbiology

Figure 5

From: A high-throughput cloning system for reverse genetics in Trypanosoma cruzi

Figure 5

Efficiency of L27 and 29A complexes purification with the original TAP tag tested in T. cruzi cells. In A, the TAP tag-fused Tcr L27 (L27), Tc pr29A (29A) and the control TAPneo-CTRL (CTRL) was detected by western blot with anti-CBP antibody. In B, the fractions from TAP purification were probed with anti-L26 and anti-╬▒2 in immunoblots. Lanes represent total protein (T) or eluted product after digestion (E). BenchMark (Invitrogen) was used as the molecular weight marker.

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