Figure 5
From: A high-throughput cloning system for reverse genetics in Trypanosoma cruzi
Efficiency of L27 and 29A complexes purification with the original TAP tag tested in T. cruzi cells. In A, the TAP tag-fused Tcr L27 (L27), Tc pr29A (29A) and the control TAPneo-CTRL (CTRL) was detected by western blot with anti-CBP antibody. In B, the fractions from TAP purification were probed with anti-L26 and anti-α2 in immunoblots. Lanes represent total protein (T) or eluted product after digestion (E). BenchMark (Invitrogen) was used as the molecular weight marker.
