Figure 1
From: A high-throughput cloning system for reverse genetics in Trypanosoma cruzi
Southern blot analysis of transfected T. cruzi cells. Lanes represent Hind III-digested: genomic DNA from T. cruzi wild type (WT), from T. cruzi transfected with the TAPneo-Tc pr29A plasmid (29A) and TAPneo-Tc pr29A isolated plasmid (Control). The neomycin resistance marker (NEO) and the tandem affinity purification tag (TAP) were used as probes. 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker.