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Table 2 Oligonucleotides used in this study

From: Strain-specific differences in pili formation and the interaction of Corynebacterium diphtheriae with host cells

Designation Sequence (5'→3' direction) Application
DIP0235-as ctt ggt tgc cgg agc agc ctc ctt PCR spaD
DIP0235-s ccg aaa cca aga ccg aga aga ccg tca ag PCR spaD
DIP0237-as gca cac cag tca gcg cca agt cgc PCR spaE
DIP0237-s cgc gac tac gga acc gac acg ctg a PCR spaE
DIP0238-as gaa gtt gaa agg tcg gcc act aca gca a PCR spaF
DIP0238-s aaa ggg cta cta cat caa cat tcc aga cac PCR spaF
DIP2010asT7 cgc gta ata cga ctc act ata ggg tcc atc acg agg aac gac aac ggt ttt aga spaC probe
DIP2010-s cgc tac tcc tat ggg caa gca cct act gat att c spaC probe
DIP2011asT7 cgc gta ata cga ctc act ata ggg tgg tgg cga tgg cca gca gtc cga spaB probe
DIP2011-s tgc agc att cgc cga cga cca acc spaB probe
SondeDIP2013-asT7 ggg ccc taa tac gac tca cta tag gga gga ctg gag tgt tgc gcc g spaA probe
SondeDIP2013-s ggc gtc gaa aat caa gct gg spaA probe
DIP2223asT7 cgc gta ata cga ctc act ata ggg atc ggt aac ttc ctt acg gaa ctt ctc tgg cag spaI probe
DIP2223-s ttg ccc gcg gga act atc gac gga spaI probe
SondeDIP2226-asT7 ggg ccc taa tac gac tca cta tag ggc cca gcc cct gcg acg tc spaH probe
SondeDIP2226-s gga ggg ctg gga ggc agt ca spaH probe
DIP2227asT7 cgc gta ata cga ctc act ata ggg tcg acc ttg gac cag tgg acc tta gcg spaG probe
DIP2227-s ccg gac aga aga ttg ctg ccg agg ca spaG probe
PromDIP2223-as cgc ggg atc cag tag ggc gtc ctt tca gga construction reporter plasmid for spaI
PromDIP2223-s cgc gat gca tgt gac gcc attt tat gta cgc construction reporter plasmid for spaI
PromDIP2226-as cgc ggg atc cag ggt gtt ttc ctt tca gga construction reporter plasmid for spaH
PromDIP2226-s cgc gat gca tcg tca aag tta cgg ccg acc construction reporter plasmid for spaH
PromDIP2227-as cgc ggg atc cag tga aaa cac ctt cta ggg construction reporter plasmid for spaG
PromDIP2227-s cgc gat gca ttg aac cgg aat cat ttc tta construction reporter plasmid for spaG
PromDIP2013-neu-as cgc ggg atc ccc ctc aac tta ttt att tgg caa aaa g construction reporter plasmid for spaA
PromDIP2013-neu-s cgc gat gca tat tgg tga gac tac ttc ctt aaa gct ggt construction reporter plasmid for spaA