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Table 3 Bacterial strains and plasmids used in this study

From: Involvement of EupR, a response regulator of the NarL/FixJ family, in the control of the uptake of the compatible solutes ectoines by the halophilic bacterium Chromohalobacter salexigens

Strain or plasmid Relevant genotype and/or description Source or reference
C. salexigens strains   
   DSM 3043T Wild type [19]
   CHR61 Spontaneous Rfr mutant of C. salexigens DSM 3043 [21]
   CHR95 CHR61 ΔeupRmntR::Tn1732; Rfr Kmr This study
   CHR161 CHR61 mntR::Ω; Rfr Smr Spcr This study
   CHR183 CHR61 eupR::Ω; Rfr Gnr This study
E. coli strain   
   DH5α supE44 Δ(lac)U169 ϕ80dlacZ ΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1; host for DNA manipulations [65]
Plasmids   
   pKS(-) Cloning vector; Apr Stratagene
   pHP45Ω pBR322 derivative carrying the Ω cassette; Apr Smr Spr [50]
   pHP45Ωaac pBR322 derivative carrying the Ωaac cassette; Apr Gmr Gnr [51]
   pRK600 Helper plasmid; Cmr tra [66]
   pJQ200-SK Suicide vector; Gmr mob sac [52]
   pSUP102-Gm::Tn1732 Mutagenesis plasmid carrying Tn1732; Cmr Kmr Gmr [40, 49]
   pRR1 pKS derivative carrying a 20.8-kb sac I fragment from CHR95 including Tn1732; Apr Kmr This study
   pMntREupR 3-kb Xba I-Apa I fragment from C. salexigens genome (containing orf1, eupR, mntR, orf4) cloned into pKS; Apr This study
   pHpaIMntr pMntREupR derivative containing a Hpa I recognition site within mntR; Apr This study
   pHindIIIEupR pMntREupR derivative containing a Hin dIII recognition site within eupR; Apr This study
   PΩMntR pHpaIMntr derivative with Ω cassette within mntR; Apr Smr Spr This study
   pΩEupR pHindIIIEupR derivative with Ωaac cassette within eupR; Apr Gnr Gmr This study
   pJQMntR 5-kb Xba I-Apa I fragment from pΩMntR (containing orf1, eupR, mntR::Ω, orf4) cloned into pJQ200-SK; Gmr Smr Spr This study
   pJQEupR 5-kb Xba I-Apa I fragment from pΩEupR (containing orf1, eupR::Ωaac, mntR, orf4) cloned into pJQ200-SK; Gmr Gnr This study