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Table 1 Sample list

From: Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method

ID Barcode PCR conditions Read number Chao1 Ace
   T* C& E$ (total) (unique) (unique) 0.03 (unique) 0.03
A1 TGGAGTAG 1 30 Ex 83,194 17,841 58,148 13,020 108,316 18,590
A2 TGTGACTG 1 30 Ex 158,519 30,361 55,899 34,096 107,984 22,871
B1 CAGACAGA 20 30 Ex 52,793 12,874 39,159 7,455 69,614 9,274
B2 CAGTGAGA 20 30 Ex 78,392 16,846 50,838 8,986 88,268 10,782
C1 CATCTCGT 200 30 Ex 25,705 6,013 16,586 2,700 24,554 2,669
C2 GGTAGGAT 200 30 Ex 25,514 5,968 16,828 2,731 25,294 2,649
D1 GTGTAGAG 20 25 Ex 10,833 3,992 13,749 4,457 26,155 6,406
D2 GTTGGTAC 20 25 Ex 25,181 7,578 22,921 6,698 42,784 9,517
E1 GTCAGAGA 20 30 Pfu 34,600 6,750 17,853 6,332 30,589 9,255
E2 GTCTTCTG 20 30 Pfu 35,152 6,818 18,281 6,416 30,434 8,792
  Total     529,883 67,826 229,287 34,883 120,750 50,579
  1. *: Dilution folds of the DNA template; &: PCR cycle number; $: Polymerase used (Ex, Ex Taq from Takara; Pfu, PfuUltra II Hotstart 2× Master Mix from Stratagene).