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Figure 5 | BMC Microbiology

Figure 5

From: Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U

Figure 5

SDS-PAGE analysis of PHA granules isolated in different growth phases. Lanes: Molecular weight marker (kD, lane 1), PHA granules isolated from P. putida U after 8 hours (lane 2), 14 hours (lane 3), 20 hours (lane 4) and 25 hours (lane 5) of growth on octanoate. Increasing amounts of PHA granules were applied: 0.1 mg (lane 2), 0.5 mg (lane 3), 1 mg (lane 4) and 1.5 mg (lane 5), respectively. Experiments were performed three times. For different cultivations, the absolute values regarding total amount of PHA granule-attached proteins had variations due to sample taken at different time points; however, PHA reganule-attached proteins exhibited similar pattern relative to cell growth in these three experiments. In this study, only the results obtained from one experiment were presented.

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BMC Microbiology

ISSN: 1471-2180

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