Figure 4

Western blot analysis of PhaC1 in P. putida U harvested at different growth stages. P. putida U was grown on 15 mM octanoate in nitrogen limited medium (0.2 NE2). Antibodies specific against PhaC1 were used to follow PhaC1 levels in P. putida U cells grown on octanoate and harvested after 8 (lane 1), 14 (lane 2) and 25 hours (lane 3). All lanes were loaded with an equal amount of cellular protein (20 μg).